| Primer sequences and properties of 22 SSR markers in Douglas-fir. | |||
| Locus | Forward primer, 5’-3’ | Reverse primer, 5’-3’ | GenBank accession |
| PmOSU_1C3 | CTCCCCTCCAGATTTTACTC | TGGCGTAACAAATAAGAGAAA | AY485312 |
| PmOSU_1F9 | CCTCATGCATTGGACACTC | GGATTCTTGAGCAGGTAGG | AY485313 |
| PmOSU_2C2 | TAAATCCGCAGCTCATAGAATC | GGGTGGTGGCTAGGGAAAC | AY485314 |
| PmOSU_2C3 | AAAGACAACATTATGAAAGG | GTAATGGTTCGAAAAATAATG | AY485315 |
| PmOSU_2D4 | TTATTGCACATGAGTATTATGA | CAGATGTTGTTTTTTATACCAC | AY485316 |
| PmOSU_2D6 | GGAAAATATACATCTCACGAC | AAGCATGCGTACTAGGTG | AY485317 |
| PmOSU_2D9 | TCGATTTACGCTTTTTTCTC | TGTTTATCCCCAGTCTCAAG | AY485318 |
| PmOSU_2G12 | CAAGGACTCATATGGGAAA | AACATCAGTAATAACCTTTT | AY485319 |
| PmOSU_3B2 | CTTTGGAGTTCTTAATATAG | GATAATAGCACCCCACCATA | AY485320 |
| PmOSU_3B9 | TGTGTAAAAATGTCTAATCC | ACTACTATTCGAGGTTTTCT | AY485321 |
| PmOSU_3D5 | GGCATCCTATTTTTCATTTT | GTGATTACCTAACTTGTGC | AY485322 |
| PmOSU_3F1 | GACTAGATCATCGCAACTT | GGTATTCTTATGGTTTTTAT | AY485323 |
| PmOSU_3G9 | ATTCCTTTTGAGACCTACTT | CTTCAAAAATTCCTACAACA | AY485324 |
| PmOSU_4A7 | TTGTAAAAATTCCCATGTAT | AAGTGGGGGAGTGTGTAAT | AY485325 |
| PmOSU_4G2 | ATTTTTTGTATTGTGCTTG | TGGATATATTTGCATTTTAC | AY485326 |
| PmOSU_2B6 | TTGTTGGGTATAATTTTCA | TAATAAAATAGCTCTAACCC | AY485327 |
| PmOSU_2G4 | ATGCATTCTTGAAAGTAAA | ATAATATGCAAGTGAATCCC | AY485328 |
| PmOSU_3E3 | TGCTTCAATTTCATATCTA | TAACATTTCAATCTATTCAC | AY485329 |
| PmOSU_3H4 | TTTGCCGTCACATTTTTATTG | GCATCTTTCAGGCATAGTCT | AY485330 |
| PmOSU_4E9 | GTTGGTTGTGTATATTCAGTTT | GCCTCTTCTTGGTTTGGT | AY485331 |
| PmOSU_5A8 | CATTTTTGGATTCTGGTTTTG | ATGCCTCAAGCTATGTAATC | AY485332 |
| PmOSU_783 | GAGCTGATGCCTTGAAGACT | CAAGTCAGTTCACAATTCCT | AA701783 |