1. 1994. Annual Report - Forest Research Laboratory, Oregon State University. Forest Research Laboratory, Oregon State University. ii + 29 pp.

Keywords:      genetic tree improvement

                        tree/stand protection    

                        tree phenology

                        tree physiology     

Abstract: Highlights of research conducted during 1993-1994 are presented, including: preliminary results of a 2-year (1992-94) field cold hardiness study of Douglas fir [Pseudotsuga menziesii] in Oregon, USA involving open pollinated progeny of 40 parents at a high and a low elevation; results of a progeny test on the frequency of second flushing of Douglas fir near Orleans, France; and variation in stable carbon isotope ratios (a measure of water use efficiency) among varieties and populations (coastal and Rocky Mountain) of Douglas fir.


2.
1997. PNWTIRC Annual Report 1996-97, Pacific Northwest Tree Improvement Research Cooperative. Oregon State University, Oregon, USA. ii + 29 p.

Keywords:      genetic tree improvement

                        tree/stand protection     

                        tree phenology

                        growth

                        wood quality

                        tree physiology

Abstract: The report describes highlights for 1996-97, current research (3 projects), student project updates (3 projects), planned Douglas fir [Pseudotsuga menziesii] seed orchards for the new millennium and other planned activities of the PNWTIRC, a research cooperative operating in the Pacific Northwest area of North America (USA and Canada). Details are included of publications and finances. Details of the 3 current research projects and the 3 student projects, which all concern Douglas fir, are presented as short papers including brief results: (1) Influence of second flushing on cold hardiness; (2) Seedling drought physiology study; and (3) Quantitative trait loci influencing cold hardiness; (4) Seedling cold hardiness; (5) Growth response of saplings to drought; and (6) Measurement study follow-up: age-age correlations in forking defects.

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3.
2000. Annual Report 1999/2000 - Pacific Northwest Tree Improvement Research Cooperative. Oregon State University, Oregon, USA. 31 p.

Keywords:      genetic tree improvement

                        tree/stand protection

                        tree physiology

                        reproduction

Abstract: Includes highlights of 1998-1999; a note to the cooperative members from Tom Adams; Introduction; Current research on seedling drought physiology of Douglas fir [Pseudotsuga menziesii], field drought study - genetics of drought sensitivity in older trees, early testing revisited, miniaturized orchard study, pollen contamination study; activities planned for 2000-2001; list of staff publications and abstracts; and a summary of financial support for the fiscal year 1999-2000.

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4.
2001. Common insects and diseases of interior Douglas-fir. British Columbia Ministry of Forests SIL471. 8 p.

Keywords:      tree/stand protection

                        tree/stand health

Abstract: This field guide provides information on the different pests and diseases of the interior Douglas fir (Pseudotsuga menziesii) in British Columbia which include: defoliating insects (Douglas fir tussock moth, spruce budworm and rusty tussock moth); dwarf mistletoe; foliar diseases (e.g., caused by the Cooley spruce adelgid); bark beetles; root diseases (Armillaria root disease, blackstain root disease, laminated root rot and blackstain root disease); wood decay (caused by bracket or conk fungi); and various abiotic problems (sunscald, drought or frost). A guideline to control infestations of these given pests is also included.

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5.
Adams, T., T. Anekonda and C. Lomas. 1999. Annual Report 1998-99, Pacific Northwest Tree Improvement Research Cooperative. 33 p.

Keywords:      genetic tree improvement

                        tree/stand protection

                        tree/stand health

                        growth

                        tree physiology

Abstract: Summaries are given of research projects on improvement of Douglas fir [Pseudotsuga menziesii] in the Pacific Northwest: seedling drought physiology; genetics of dark respiration and its relationship with drought hardiness; response of saplings to drought, as measured by growth ring variables; use of microsatellite marker loci to identify pollen contamination in seed orchards; and evaluation of miniaturized seed orchard designs.

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6.
Aitken, S.N. and W.T. Adams. 1996. Genetics of fall and winter cold hardiness of coastal Douglas-fir in Oregon. Canadian-Journal-of-Forest-Research 26(10): 1828-1837.

Keywords:      genetic tree improvement

                        tree/stand protection

                        tree/stand health

                        tree physiology

                        genetic relationships

Abstract: Genetic variation in autumn cold hardiness was studied in two western Oregon breeding populations of coastal Douglas fir (Pseudotsuga menziesii var. menziesii), one on the west slope of the Cascade Mountains and the other in the Coastal Range. On six sampling dates (September, October and November 1992 and January, September and October 1993), shoot cuttings from 40 open-pollinated families in each of two progeny test sites for each breeding zone were subject to artificial freezing at two test temperatures. Damage in each shoot was recorded as visible injury to needle, stem and bud tissue separately. Considerable family variation was found for cold injury scores in all tissues in early to mid autumn, but differences were often smaller or nonsignificant in late autumn and midwinter. Individual heritability estimates for needle cold injury were low (<0.40) and generally decreased in late autumn and midwinter. Family rankings for autumn cold hardiness, however, are expected to be relatively consistent over sites and years, although needles appear to display more family-by-site interaction than stems or buds. Genetic correlations between tissues in cold injury varied considerably and were sometimes weak, indicating that the evaluation of a single tissue is probably not adequate for assessing overall cold hardiness of genotypes. Autumn and winter cold hardiness seem to be largely under separate genetic control since genetic correlations between hardiness at these two stages were weak. This study confirms earlier results in Washington breeding populations and shows that coastal Douglas fir families can be effectively ranked for autumn cold hardiness by conducting artificial freeze tests on cut shoots in mid-autumn (October) and scoring damage to stems and at least one other tissue.

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7.
Aitken, S.N. and W.T. Adams. 1997. Spring cold hardiness under strong genetic control in
Oregon populations of Pseudotsuga menziesii var. menziesii. Canadian-Journal-of-Forest-Research 27(11): 1773-1780.

Keywords:      genetic tree improvement

                        tree/stand protection

                        tree/stand health

                        tree phenology

                        tree physiology

                        genetic relationships

Abstract: Genetic variation in spring cold hardiness of shoots prior to bud break was studied in two Oregon breeding populations of Pseudotsuga menziesii var. menziesii, one on the west slope of the Cascade Mountains and the other in the Coast Range. In March and April 1993, and April 1994, shoot cuttings from 40 open-pollinated families in each of two progeny test sites in each breeding zone were subjected to artificial freezing. Visible cold damage to needle, stem, and bud tissues was recorded. Date of bud burst (all sites), and injury resulting from a 1992 natural frost event (one site), were also recorded. Spring cold injury varied widely among families. Individual heritabilities for spring cold injury scores averaged 0.76 in the Coastal zone and 0.42 in the Cascade zone. Genetic correlations among tissues, sites, sampling dates, and years, and between April cold injury and date of bud burst were high, in most cases over 0.80. Correlations were also strong between natural frost damage in 1992 and artificial cold injury scores in 1993. Artificial freeze testing stem tissues of cut shoots sampled in April from a single test site should effectively rank families in this region for spring cold hardiness.

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8.
Aitken, S.N., W.T. Adams, N. Schermann and L.H. Fuchigami. 1996. Family variation for fall cold hardiness in two Washington populations of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii (Mirb.) Franco). Forest-Ecology-and-Management 80(1/3): 187-195.

Keywords:      genetic tree improvement

                        tree/stand protection

                        tree/stand health

                        tree physiology

                        tree phenology

                        genetic relationships

Abstract: In order to assess the genetics of autumn (fall) cold hardiness in coastal Douglas fir (Pseudotsuga menziesii var. menziesii), shoot cuttings were collected in October from saplings (9-year-old trees) of open-pollinated families in two progeny tests in each of two breeding zones in Washington, one in the Coast range (80 families) and one on the west slope of the Cascade Mountains (89 families). Samples from over 5500 trees were subjected to artificial freezing and visually evaluated for needle, stem and bud tissue injury. The extent to which cold injury is genetically related to tree height and shoot phenology (timing of bud burst and bud set) was also evaluated. Significant family variation was found for all cold hardiness traits; however, individual heritability estimates were relatively low (ranging from 0.09 to 0.22). Significant family-by-test site interaction was detected for needle injury in the Cascade breeding zone, but not in the coastal zone. Genetic correlations (rA) among needle, stem and bud tissues for cold damage were weak (0.16<less or =>rA<less or =>0.58) indicating that genes controlling autumn cold hardening are somewhat different for different tissues. Timing of bud burst and bud set were only weakly correlated with cold injury (rA<less or =>0.49). Thus, bud phenology is a poor predictor of autumn cold hardiness in this species. There was no consistent relationship between tree height and cold injury in the coastal zone. In the Cascade zone, taller trees appeared to be more susceptible to cold injury, but the association was weak (mean rA=0.38, range 0.20-0.72).

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9.
Anekonda, T.S., M.C. Lomas, W.T. Adams, K.L. Kavanagh and S.N. Aitken. 2002. Genetic variation in drought hardiness of coastal Douglas-fir seedlings from British Columbia. Canadian-Journal-of-Forest-Research 32(10): 1701-1716.

Keywords:      genetic tree improvement

                        tree/stand protection

                        growth

                        tree/stand health

                        genetic relationships

                        tree physiology

Abstract: Genetic variation in drought hardiness traits and their genetic correlations with growth potential and recovery traits were investigated in 39 full-sib families of coastal Douglas-fir (Pseudotsuga menziesii var. menziesii) from southwestern British Columbia, Canada. Seedlings of these families were grown in raised nursery beds and subjected to three moisture regimes each in the second (well-watered or control, mild, and moderate drought) and third (control, severe drought, and recovery from second-year moderate drought) seasons. Traits assessed included drought hardiness (foliage damage, cavitation of xylem tracheids, xylem hydraulic conductivity, and height and diameter growth increment) in the drought treatments, growth potential (total height and diameter) in the control treatment, and height and diameter growth increments in the recovery treatment. Xylem cavitation in the growth ring produced in a particular year was nearly three times greater under the moderate drought and four times greater under the severe drought than in the control treatment. Xylem hydraulic conductivity of seedlings in the severe drought treatment was 40% lower than conductivity of seedlings under the control treatment. Mean foliage damage in seedlings subjected to severe drought (third season) was much greater (33%) than in seedlings subjected to mild or moderate drought (second season). Families differed significantly in most drought hardiness traits, with individual tree heritabilities averaging 0.19. Thus, much potential exists for identifying drought-hardy families at the seedling stage and using this information for deployment or breeding purposes. In addition, most hardiness traits were strongly intercorrelated (genetic correlations often exceeded |0.80|) indicating that these traits are controlled largely by the same set of genes and that selection for hardiness based on one trait will increase hardiness as reflected in the other traits as well. Genetic correlations were only moderate (0.49) between hardiness traits measured in different years, perhaps due to the large difference in severity of the drought applied in the two seasons. Although injury to seedlings, as reflected in foliage damage and xylem cavitation, was relatively low under the moderate drought of the second season, it did result in reduced growth increment the following (recovery) year. Growth potential under favourable moisture regimes was nearly uncorrelated with drought hardiness, suggesting that drought hardiness could be improved in this southwestern British Columbia breeding population without negatively impacting growth potential in favourable moisture conditions.

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10.
Antonelli, A.L. and R.L. Campbell. 1991. Cooley spruce gall aphid. College of Agriculture and Home Economics, Washington State University. Extension Bulletin EB0966: 2 p.

Keywords:      tree/stand protection

                        tree/stand health

Abstract: Notes are provided on the biology, injuriousness and chemical control (carbaryl and endosulfan are suggested) of Adelges cooleyi [Gilletteella cooleyi] on certain coniferous trees [including Picea sitchensis, P. engelmannii, P. pungens and Pseudotsuga menziesii] in Washington State.

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11.
Axelrood, P.E., W.K. Chapman, K.A. Seifert, D.B. Trotter and G. Shrimpton. 1998. Cylindrocarpon and Fusarium root colonization of Douglas-fir seedlings from British Columbia reforestation sites. Canadian Journal of Forest Research 28:1198-1206.

Keywords:      planting operations

                        tree/stand protection

                        tree/stand health

Abstract: Poor performance of Douglas fir (Pseudotsuga menziesii) plantations established in 1987 has occurred in southwestern British Columbia. Affected sites were planted with 1-yr-old container stock that exhibited some root dieback in the nursery. A study was initiated in 1991 to assess Cylindrocarpon and Fusarium root infection in planted and naturally regenerating (natural) Douglas fir seedlings from 7 affected plantations. Percentages of seedlings harbouring Cylindrocarpon spp. and percentage root colonization were significantly greater for planted seedlings than natural seedlings. A significant linear trend in Cylindrocarpon root colonization was observed for planted seedlings with colonization levels being highest for roots closest to the remnants of the root plug and decreasing at distances greater than 10 cm from that region. This trend in Cylindrocarpon colonization was not observed for natural seedlings. Cylindrocarpon destructans var. destructans [Nectria radicola var., radicola] and C. cylindroides var. cylindroides were the only species isolated from planted and natural conifer seedlings. For most sites, percentage of seedlings harbouring Fusarium spp. and percentage Fusarium root colonization were less than for Cylindrocarpon. Recovery of Fusarium spp. from seedlings and root colonization levels were not significantly different for planted and natural seedlings from all sites.

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12.
Axelrood, P.E., M. Neumann, D. Trotter, R. Radley, G. Shrimpton and J. Dennis. 1995. Seedborne Fusarium on Douglas-fir: pathogenicity and seed stratification method to decrease Fusarium contamination. New-Forests 9(1): 35-51.

Keywords:      nursery operations

                        tree/stand protection

                        tree/stand health

                        growth

Abstract: Twelve Douglas-fir (Pseudotsuga menziesii) seedlots from coastal British Columbia were assayed for seedborne Fusarium; all of the seedlots were contaminated. The percentage of non-stratified seeds from individual seedlots harbouring Fusarium ranged from 0.3 to 95.4. Sixty-seven percent of the seedlots had Fusarium on less than 2% of the seeds. Post-stratification seedborne Fusarium levels were significantly less for running water imbibition compared with standing water imbibition. However, seedling growth at a container nursery was not consistently different for stratified seed imbibed initially in standing or running water. Fusarium disease symptoms were not observed in the nursery environment. The species of Fusarium isolated from seed were F. acuminatum [Gibberella acuminata], F. avenaceum [G. avenacea], F. lateritium [G. baccata], F. moniliforme [G. fujikuroi], F. oxysporum, F. poae and F. sambucinum [G. pulicaris]. Twelve Fusarium isolates, comprising 6 species, were assessed for pathogenicity. Disease symptoms were observed after 4 weeks incubation and Fusarium isolates ranged in virulence from low to high. Fusarium oxysporum isolates were the most pathogenic.

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13.
Axelrood, P.E. and R. Radley. 1991. Biological control of Fusarium on Douglas-fir seedlings. Bulletin-SROP 14(8): 85-87.

Keywords:      nursery operations

                        tree/stand protection

                        tree/stand health

Abstract: A bacterial culture collection was established from the rhizosphere and rhizoplane of Douglas fir (Pseudotsuga menziesii) seedlings collected from nursery and forest locations in British Columbia, Canada. Of the 2000 strains screened, 350 inhibited growth of at least 1 conifer seedling root pathogen (Fusarium, Cylindrocarpon or Pythium) in in vitro antibiosis assays. A total of 96 strains were screened for Fusarium disease control in biological control assays. One strain that inhibited all 3 pathogens in vitro was able to significantly reduce the incidence of disease caused by Fusarium on P. menziesii seedlings. Another strain that tested negative in in vitro antibiosis assays also reduced the disease incidence by a similar amount. This paper was presented at the Second international workshop on plant growth-promoting rhizobacteria - progress and prospects, held in Interlaken, Switzerland, Oct. 14-19, 1990.


14.
Bloomberg, W.J. 1988. Modeling control strategies for laminated root rot in managed Douglas-fir stands: model development. Phytopathology 78(4): 403-409.

Keywords:      planting operations

                        site preparation

                        mechanical preparation

                        tree/stand protection

                        tree/stand health

Abstract: A model of laminated root rot caused by Phellinus [Inonotus] weirii was developed to assess potential control strategies in managed Pseudotsuga menziesii stands. The model mimicked key processes in disease initiation and development quantified as functions of time and space. These processes were horizontal and vertical tree root distribution, root contact with inoculum and among root systems, spread of mycelium through root systems, root decay, reduction of diam. growth in infected trees, tree mortality and persistence of inoculum in roots of stumps and killed trees. The processes were expressed as mathematical functions which were integrated in a computer program to calculate spread of the disease and stand-growth loss and mortality. Data for quantification of functions were obtained by experiments and from the literature. Simulated control practices included infected stump removal, sanitation fellings and mixed planting of Douglas fir and resistant species. Accuracy of the model was tested by comparing calculated disease spread and mortality with the following data: (1) spread and damage in two 60-yr-old, 1-ha stands in Oregon, (2) results from a statistically based model for spread and damage that had performed satisfactorily, and (3) observed spread and damage behaviour in stands of different ages and growth rates. Results from the model compared favourably with all of the above situations.

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15.
Bloomberg, W.J. and G. Reynolds. 1988. Equipment trials for uprooting root-rot-infected stumps. Western-Journal-of-Applied-Forestry 3(3): 80-82.

Keywords:      site preparation

                        mechanical preparation

                        tree/stand protection

                        tree/stand health

Abstract: Residual roots from Douglas fir (Pseudotsuga menziesii) and western hemlock (Tsuga heterophylla) were measured following stump-root extraction one yr after harvesting a 55-yr-old, 314 stems/ha, 47% Douglas fir, 17% maple (Acer macrophyllum), 16% red cedar (Thuja plicata), 6% western hemlock stand with 20% infection by Phellinus weirii in the Cowichan valley, Vancouver Island, Canada. Extraction was by a Caterpillar D8H with brush-clearing blade, a 180-hp backhoe or a 115-hp backhoe. All 3 machines recovered more than 90% of root vol. The small backhoe left significantly greater numbers and lengths of root residues per msuperscript 3 soil, though the vol. of residues was greatest for the Caterpillar. An earlier study suggested that a root density of 32 roots/msuperscript 3 was needed to produce one root contact; as the least efficient treatment by the Caterpillar left 23.2 roots/msuperscript 3 in the ground, it is suggested that this would provide insufficient contacts with a new tree crop to transmit infection.

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16.
Brandeis, T.J., M. Newton and E.C. Cole. 2002. Biotic injuries on conifer seedlings planted in forest understory environments. New Forests 24:1-14.

Keywords:      planting operations

                        site preparation

                        chemical preparation

                        release treatments

                        chemical release

                        thinning

                        tree/stand protection

                        growth

                        tree/stand health

Abstract: The effects of partial overstorey retention, understorey vegetation management, and protective Vexar(R) tubing on the frequency and severity of biotic injuries in a two-storied stand underplanted with western redcedar (Thuja plicata), Douglas-fir (Pseudotsuga menziesii), grand fir (Abies grandis), and western hemlock (Tsuga heterophylla) were investigated. The most prevalent source of damage was browsing by black-tailed deer (Odocoileus hemionis columbiana); deer browsed over 74% of Douglas-fir and over 36% of western redcedar seedlings one or more times over the four years of this study. Neither the spatial pattern of thinning (even or uneven) nor the density of residual overstorey affected browsing frequency. Spraying subplots may have slightly increased browsing frequency, but the resulting reduction of the adjacent understorey vegetation increased the volume of all seedlings by 13%, whether or not they were browsed. Vexar(R) tubing did not substantially affect seedling survival, browsing damage frequency, or fourth-year volume. Greater levels of overstorey retention reduced frequency of second flushing. Chafing by deer and girdling by rodents and other small mammals began once seedlings surpassed 1 m in height. Essentially all grand fir seedlings exhibited a foliar fungus infection.

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17.
Campbell, D.L. and J. Evans. 1988. Recent approaches to controlling mountain beavers (Aplodontia rufa) in Pacific Northwest forests. In Proceedings: Thirteenth Vertebrate Pest Conference, Monterey, California. pp. 183-187.

Keywords:      tree/stand protection

                        tree/stand health

Abstract: Investigations by the Denver Wildlife Research Center into ways of managing mountain beaver (Aplodontia rufa) populations are described. Methods were developed for alleviating mountain beaver damage to conifer trees being grown for timber in the Pacific Northwest. Studies initiated in 1986 indicated that aversive conditioning with Big Game Repellent Powder (BGR-P) dusted on culled Douglas fir (Pseudotsuga menziesii) seedlings placed in burrows significantly reduced mountain beaver damage to planted seedlings treated with BGR-P and to untreated seedlings. Trials also showed that strychnine-sword fern (Polystichum munitum) baits prepared with a 4.9% (active) strychnine paste concentrate were very effective and selective for mountain beaver control. Other topics discussed are the results of several probes with toxic baits and phosphine gas, trials with a drug (reserpine) and a wetting agent to induce hypothermia, and destruction of underground nests to prevent reinvasion.

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18.
Campbell, S.J. and P.B. Hamm. 1989. Susceptibility of Pacific Northwest conifers to Phytophthora root rot. Tree Planters' Notes 40(1): 15-18.

Keywords:      nursery operations

                        tree/stand protection

                        tree/stand health

Abstract: One-yr-old bare-rooted seedlings of 11 conifer species were inoculated with (a) Phytophthora cactorum, (b) P. cryptogea, (c) P. drechsleri, (d) P. megasperma or (e) P. pseudotsugae. Development of above-ground symptoms and root disease was followed for 10 wk. Isolates of (a), (b) and (e) caused the most overall mortality and isolates of (d) the least. Pinus contorta, P. ponderosa, P. monticola, Larix occidentalis, Libocedrus decurrens, Picea sitchensis and P. engelmannii showed tolerance to the root disease, Abies grandis and Pseudotsuga menziesii showed intermediate susceptibility and A. magnifica and Tsuga mertensiana were quite susceptible. Results are discussed in relation to management of conifer nurseries in the Pacific Northwest.

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19.
Carroll, G.C. 1988. Facultative fungal egg-parasites as agents of gypsy moth mortality. Northwest-Environmental-Journal 4(2): 345-346.

Keywords:      tree/stand protection

                        tree/stand health

Abstract: Research on entomogenous fungi attacking the forest pest Lymantria dispar in Oregon is summarized. The pathogenicity of 20 fungi was tested against eggs in the laboratory and Beauveria bassiana, Paecilomyces farinosus, Spicaria coccospora and Verticillium lecanii were shown to be consistently pathogenic. Preliminary studies showed that some of these fungi can invade and persist in bark of Douglas fir [Pseudotsuga menziesii] and oak [Quercus spp.], and subsequently infect egg masses on the bark.

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20.
Chanway, C.P. 1997. Inoculation of tree roots with plant growth promoting soil bacteria: an emerging technology for reforestation. Forest-Science 43(1): 99-112.

Keywords:      nursery operations
tree/stand protection
growth

                        tree/stand health

                        mycorrhizal response

Abstract: Results from studies performed with beneficial asymbiotic tree root associated bacteria are reviewed in this article in relation to the possible uses of such microorganisms for artificial forest regeneration. The review includes sections on plant growth promoting bacteria for pine (Pinus spp.), spruce (Picea spp.), Douglas fir (Pseudotsuga menziesii) and hemlock (Tsuga heterophylla). Seedling root systems are colonized heavily by asymbiotic soil bacteria, many of which have the potential to influence plant growth significantly. A heterogeneous group of these microorganisms is well known for their ability to colonize roots and stimulate growth of agricultural plant species, sometimes doubling seedling biomass accumulation only a few weeks after inoculation, but more usually resulting in less spectacular biomass gains (e.g., 15%-30% greater than uninoculated controls within a growing season). Plant growth promoting soil bacteria may exert such effects through a variety of mechanisms, and include microorganisms that stimulate seedling emergence or infection by symbiotic fungi and bacteria. Other plant beneficial soil bacteria possess biological control activity or are capable of transforming plants genetically. Inoculation of tree seedlings with such bacterial before outplanting would be an inexpensive, environmentally benign, and easily applied nursery treatment, but comparatively little work has been performed with these microorganisms in forestry. Recent results with various tree species, however, indicate that seedling performance can be significantly enhanced through bacterial inoculation of root systems: pine and spruce biomass increased 32%-49% 1 yr after inoculation and outplanting at a reforestation site. In addition, infection by desired species of ectomycorrhizal fungi can also be enhanced by inoculation with certain strains of root colonizing bacteria.

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21.
Chastagner, G.A., R.S. Byther, J.D. MacDonald and E. Michaels. 1984. Impact of Swiss needle cast on postharvest hydration and needle retention of Douglas-fir Christmas trees. Plant-Disease 68(3): 192-195.

Keywords:      tree/stand protection

                        tree/stand health

                        tree physiology

Abstract: Healthy Douglas-fir (Pseudotsuga menziesii) Christmas trees were compared with those infected by Phaeocryptopus gaeumannii for needle loss and dehydration after cutting. The presence of infected needles increased the rate of dehydration (as measured by changes in xylem water potential) of cut trees placed in water or left dry. Fungicide applications 1 yr before harvest significantly improved retention of 1-yr-old needles on trees displayed either wet or dry, whereas applications during the year of harvest made no difference in retention of either current-season or 1-yr-old needles.

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22.
Colangeli, A.M., L. McAuley and J.N. Owens. 1990. Seasonal occurrence of potential ice-nucleating bacteria on Douglas fir foliage and seed cones. New-Forests 4(1): 55-61.

Keywords:      seed orchard management

                        tree/stand protection

                        tree/stand health

                        reproduction

Abstract: Plant frost damage can involve interactions between certain surface bacteria and low temperatures. The bacteria contain glycoproteins, which can nucleate ice above -5 degrees C, thus making the plants on which they live more susceptible to freezing. Preliminary studies to determine if bacteria were present on Douglas fir (Pseudotsuga menziesii), and whether they exhibited ice-nucleating properties, are reported. Total bacteria and fluorescent Pseudomonas populations were monitored on buds, conelets and foliage of five trees in a Douglas fir seed orchard on Vancouver Island, Canada, in April 1986 and between October 1986 and May 1987, over periods that spanned two pollination seasons. Seasonal variation in bacterial numbers was observed, with highest numbers occurring in late winter and early spring. Bacterial populations active in ice nucleation were found. Bacterial numbers during pollination were higher in 1986 than in 1987. Conelet abortion at pollination was also higher in 1986 (55%) than in 1987 (11%). A relation may exist between bacterial populations and conelet abortion at pollination.

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23.
Colangeli, A.M., J.N. Owens and S.J. Morris. 1989. Factors affecting cone and seed production in Douglas fir. BC Ministry of Forests FRDA-Report 057. 19 p.

Keywords:      seed orchard management

                        tree/stand protection

                        reproduction

Abstract: Reduced seed yield in 1986 on 4 Pseudotsuga menziesii trees in a British Columbia seed orchard was associated with inadequate pollination, low pollen vigour or viability, embryo abortion, and early ovule abortion. A study of bacterial populations suggested that there may be a causal relationship between this factor and conelet abortion.

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24.
Copes, D.L. 1999. Breeding graft-compatible Douglas-fir rootstocks (Pseudotsuga menziesii (MIRB.) FRANCO). Silvae-Genetica 48(3/4): 188-193.

Keywords:      genetic tree improvement

                        tree grafting

                        tree/stand protection

                        growth

                        tree phenology

                        tree/stand health

                        genetic relationships

Abstract: A study encompassing 24 years was conducted to determine if a breeding programme could produce highly graft-compatible rootstocks for P. menziesii. A total of 27 trees of apparent high graft compatibility were selected and crossed to produce 226 control-pollinated families. Seedlings were grown, field planted and grafted with test scions. Graft unions from field tests were evaluated anatomically for internal symptoms of incompatibility. Average compatibility of progeny from the 226 crosses was 90.6%, compared with 65% in native populations. Breeding values were calculated for each parent by the best linear prediction (BLP) procedure. Average compatibility resulting from crossing among the top 10 parents was estimated by breeding values as 95.4%. Field-test results of progeny from 34 crosses among the 10 most compatible parents showed 96% compatibility. In addition to field-tests for graft compatibility, nursery tests of seedlings from 124 crosses were evaluated for second-year vegetative bud flush and seedling height. It was possible, while maintaining adequately high levels of graft compatibility, to breed both for resistance to spring frost damage and for increased seedling height.

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25.
DeYoe, D.R., H.R. Holbo and K. Waddell. 1986. Seedling protection from heat stress between lifting and planting. Western-Journal-of-Applied-Forestry 1(4): 124-126.

Keywords:      nursery operations

                        planting operations

                        tree/stand protection

                        tree/stand health

Abstract: Seven protective treatments were evaluated for preventing overheating of 2+0 Douglas fir seedlings in Kraft paper bags. Trials were conducted in May 1982 at Corvallis, Oregon on 3 clear days with max. air temp. of 78 degrees F and a hazy day with max. temp. 66 degrees . Seedlings were returned to cold storage (35 degrees ) overnight. Seedling temp. differed significantly between treatments. Unprotected seedlings (paper bag only) in full sun reached 89 degrees after 7 h. Green canvas caused increased heating rates and higher temp. (104 degrees after 7 h). A white sheet and a crinkled foil wrap performed no better than a paper bag alone. Canvas painted off-white reduced max. temp. to 80 degrees . Heavy shading (2% of full sun) and Mylar with white surface towards the sun were the most effective materials for preventing overheating (max. temp. 59-60 degrees ). Mylar with the silver surface facing the sun was less effective (max. temp. 71 degrees ).

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26.
Donald, D.G.M. and D.G. Simpson. 1985. Shallow conditioning and late fertilizer application effects on the quality of conifer nursery stock in British Columbia. B.C.-Ministry-of-Forests Research-Note 99. viii + 36 p.

Keywords:      nursery operations

                        nursery fertilization

                        tree/stand protection

                        tree morphology

                        tree/stand health

                        growth

Abstract: Eight trials on 2+0 stock of Picea engelmannii, P. glauca, P. sitchensis, Pinus contorta and Pseudotsuga menziesii (var. glauca and var. menziesii) in 4 nurseries were conducted to compare the effects of shallow conditioning (undercutting and wrenching at 10 cm deep) with those of the standard conditioning regime (undercutting and wrenching at 20 cm) on nursery performance, storage and field performance. The application of a complete NPK fertilizer 50 days before lifting was also evaluated. Shallow conditioning and late fertilizer application improved the root growth capacity at lifting, but could not replace cold exposure for hardening Pseudotsuga menziesii. Shallow conditioning had little effect on survival after planting and reduced initial ht. increment of all species. Application of fertilizer just before lifting improved the early growth of the trees without adversely affecting survival. Planting seedlings some 5 cm deeper than they stood in the nursery improved establishment.

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27.
Dosskey, M.G., L. Boersma and R.G. Linderman. 1993. Effect of phosphorus fertilization on water stress in Douglas fir seedlings during soil drying. Plant-and-Soil 150(1): 33-39.

Keywords:      fertilization

                        tree/stand protection

                        growth

                        tree physiology

                        photosynthesis

Abstract: A growth chamber experiment was conducted to determine if P fertilizing to enhance the P nutrition of otherwise N and P deficient Douglas fir (Pseudotsuga menziesii) seedlings reduces water stress in the seedlings during drought periods. Seedlings were grown in pasteurized mineral soil under well watered conditions and fertilized periodically with a small amount of nutrient solution containing P at three levels: 0, 20, or 50 mg/litre. By age 6 months, leaf nutrient analysis indicated that N and P were deficient in control (0 mg P/litre) seedlings. The highest level of P fertilizer, which doubled leaf P concentration, did not affect plant biomass, suggesting that N deficiency was limiting growth. When these seedlings were subjected to drought, there was no effect of P fertilizing on leaf water potential or osmotic potential. Furthermore, P fertilized seedlings had lower stomatal conductance and net photosynthesis rate. These results indicate that enhanced P nutrition, in the presence of N deficiency, does not reduce water stress in Douglas fir seedlings during drought periods.

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28.
Drew, A.P. 1983. Optimizing growth and development of 2-0 Douglas-fir seedlings by altering light intensity. Canadian-Journal-of-Forest-Research 13(3): 425-428.

Keywords:      nursery operations

                        tree/stand protection

                        growth

                        tree morphology

                        carbon allocation

Abstract: Seedlings were grown outdoors in Michigan in pots under 71% of full light the first growing season and full light the second. Another group of seedlings was given full light continuously for 2 yr. At the end of the 1st year, seedlings given initial shade had grown larger in total wt. (root + shoot) than those grown under full light. With removal of shading, the larger plants began to allocate increased dry matter to root development relative to their shoots. By the end of 2 yr, shoot/root ratios for the 2 groups were no different, yet the plants shaded in their 1st year were significantly heavier (dry wt.) By proper use of shading during development, larger 2+0 planting stock with good root development may be produced. Such stock, grown without the use of costly fertilizer, may be better suited to regeneration of droughty sites in the Pacific Northwest USA than the usual 2+0 planting stock, nursery grown under full light.

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29.
Driessche, R.v.-d. 1991a. Influence of container nursery regimes on drought resistance of seedlings following planting. I. Survival and growth. Canadian-Journal-of-Forest-Research 21(5): 555-565.

Keywords:      nursery operations

                        tree/stand protection

                        growth

                        tree morphology

                        carbon allocation

                        tree/stand health

Abstract: In a 2 year study, Douglas fir (Pseudotsuga menziesii), lodgepole pine (Pinus contorta) and white spruce (Picea glauca) seedlings, grown in Styroblock containers in a container nursery from February to July 1988, were exposed to three temperatures and three levels of drought stress applied factorially during 18 July to 29 September 1988. Mean temperatures of 13, 16 and 20 degrees C were imposed in growth chambers, in a cooled plastic house, and in an ambient plastic house, respectively. Control, medium and severe levels of drought stress were imposed in a series of eight cycles, resulting in mean xylem pressure potentials of -0.32, -0.50 and -0.99 MPa, respectively. Seedlings were kept in the ambient plastic house until January 1989, when they were lifted and cold-stored until transplanting to covered 0.5-m deep sand beds, which provided hygric, mesic, and xeric conditions for testing all species and treatments. At the end of nursery growth, an increase in nursery temperature increased height and height : diameter ratio in all species and shoot:root dry weight ratio in Douglas fir and lodgepole pine. Increase in temperature also increased the number of seedlings with large well-formed buds in white spruce, but reduced the number in Douglas fir. Drought stress reduced height and dry weight in all species and bud length in lodgepole pine. After 9 weeks in sand beds, low nursery temperature increased survival (19% for lodgepole pine and white spruce grown in the xeric bed), except for Douglas fir grown in the xeric bed. Nursery drought stress also increased survival (16% for Douglas fir and lodgepole pine in the xeric bed), but had little effect on white spruce. Low temperature and drought stress treatments that increased survival also reduced height and dry weight of lodgepole pine and white spruce after one growing season in sand beds. Survival showed significant negative correlations with height, dry weight and height:diameter and shoot : root weight ratios. Low nursery temperature continued to affect growth 16 weeks after planting, increasing relative growth rate and allometric ratio (K) of Douglas fir and reducing K of white spruce.

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30.
Driessche, R.v.-d. 1991b. Influence of container nursery regimes on drought resistance of seedlings following planting. II. Stomatal conductance, specific leaf area, and root growth capacity. Canadian-Journal-of-Forest-Research 21(5): 566-572.

Keywords:      nursery operations

                        tree/stand protection

                        tree physiology

                        tree/stand health

Abstract: Seedlings of Douglas fir (Pseudotsuga menziesii), lodgepole pine (Pinus contorta) and white spruce (Picea glauca) were grown in a container nursery from February to July 1988 and then exposed to three temperatures and three levels of drought stress applied factorially during mid-July to October 1988. Seedlings were retained in a shelter house until January 1989, when they were cold-stored until early May. Measurements of stomatal conductance (gs), transpiration (E), and specific leaf area (SLA) were made at the end of the treatment period in September 1988 and again after growth the following year at the end of June. Root growth capacity (RGC) was tested in early May 1989. Results were considered in conjunction with performance of other samples of the same plants that had been planted in sand beds in April 1989, where irrigation was regulated to provide three levels of moisture stress. Low temperature (13 degrees C) generally reduced gs and E, which were adjusted for xylem pressure potential, and SLA, in all species by the time nursery treatment was completed at the end of September. No effect of nursery temperature treatment on gs or E could be detected when new needles were measured in June and July (after 9 to 12 weeks of growth), but SLA of lodgepole pine increased with nursery temperature treatment, and SLA of white spruce decreased with treatment. RGC was higher for the 13 degrees C treatment than for the 16 and 20 degrees C treatments. Survival of outplanted seedlings was mainly inversely related to nursery temperature. Low nursery temperature reduced gs, E, and SLA and increased RGC. SLA of planted lodgepole pine increased with level of nursery drought treatment, and severe nursery drought increased gs under stress, when measured in June. No other effects of drought were detected, although drought treatment was effective in increasing survival of planted seedlings. It is suggested that other mechanisms, such as osmotic adjustment, were responsible for the results observed.

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31.
Driessche, R.v.-d. 1992b. Changes in drought resistance and rootgrowth capacity of container seedlings in response to nursery drought, nitrogen, and potassium treatments. Canadian-Journal-of-Forest-Research 22(5):740-749.

Keywords:      nursery operations

                        nursery fertilization

                        tree/stand protection

                        tree/stand health

                        growth

                        carbon allocation

                        tree physiology

Abstract: Douglas fir (Pseudotsuga menziesii), lodgepole pine (Pinus contorta), and white spruce (Picea glauca) seedlings, each represented by two seed lots, were grown in Styroblock containers in a greenhouse and plastic shelter house from February 1989 to January 1990. The seedlings were exposed to two N treatments (20 and 200 mg/litre) and three K treatments (5, 25 and 100 mg/litre) arranged factorially within three drought treatments. After winter storage, seedlings from a complete set of treatments were planted into hygric, mesic and xeric sand beds during 12-14 March. Increasing nursery drought stress increased survival of Douglas fir and lodgepole pine after planting, and high N treatment level increased survival of lodgepole pine and white spruce. Under xeric conditions, combined nursery drought and high N treatments increased survival of lodgepole pine by 33%, indicating the importance of nursery cultural regime for stock quality. Increase in nursery drought stress did not decrease seedling size by much; increase in N increased seedling size one season after planting. A positive relation between shoot : root ratio and survival in lodgepole pine and white spruce indicated that increase in N increased both shoot growth and drought resistance over the N range investigated. Only Douglas fir showed an interaction between drought and N treatment and a small response in both survival and dry weight to K application. Root growth capacity, measured at the time of planting, showed an approximate doubling in all species due to high N treatment, and was also increased in white spruce by drought stress. Survival and root growth capacity were poorly correlated, but dry-weight growth in sand beds was well correlated with root growth capacity. Shoot dry weight and percentage N in shoots measured after nursery growth were correlated with root growth capacity. Manipulation of root growth capacity by changing nursery treatment was possible without altering resistance to drought stress after planting.

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32. El Kassaby, Y.A., D.G.W. Edwards and C. Cook.
1990a. Impact of crop management practices on seed yield in a Douglas-fir seed orchard. Silvae-Genetica 39(5-6): 226-230.

Keywords:      seed orchard management

                        tree/stand protection

                        reproduction

                        tree/stand health

Abstract: The impact of two crop-management practices, supplemental mass pollination (SMP) and overhead cooling, on seed yield in a 13-yr-old Douglas fir (Pseudotsuga menziesii) seed orchard was studied in Saanichton, British Columbia. A 2x2 factorial trial of SMP/no SMP and cooling/no cooling was applied. There were no significant differences in potential seed yield per cone, average number of successful fertilizations, and average number of filled seeds per cone between cooling or SMP treatments or combinations. Results indicated that within-orchard pollen cloud was not a factor limiting seed yield. Average number of seeds infested by the Douglas fir seed wasp (Megastigmus spermotrophus) larvae was significantly (P<0.05) less when cooling was applied, indicating that the treatment was effective in disrupting the synchrony between the presence of ovipositing females and developing cones.

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33.
Entry, J.A., K. Cromack, Jr., R.G. Kelsey and N.E. Martin. 1991. Response of Douglas-fir to infection by Armillaria ostoyae after thinning or thinning plus fertilization. Phytopathology 81(6): 682-689.

Keywords:      thinning

                        fertilization

                        tree/stand protection

                        growth 

                        tree morphology

                        carbon allocation

                        tree/stand health

                        tree physiology

Abstract: Second-growth stands of Douglas-fir (Pseudotsuga menziesii) were thinned to a 5- x 5-m spacing (TT); additional plots were thinned and fertilized once with 360 kg of N (as urea)/ha (TF). An unthinned, unfertilized stand (UT) served as a control. Ten years after treatment, trees were inoculated with 2 isolates of A. ostoyae. Trees receiving the TF and TT treatments produced greater diameter growth, leaf area, and wood production/msuperscript 2 leaf area per year than did those under the UT treatment. Rates of infection by A. ostoyae were highest in trees that received the TF and lowest in trees that received the TT treatment. Concn of sugar, starch and cellulose in root bark tissue were highest in trees receiving the TF treatment and lowest in trees receiving TT treatment. Concn of lignin, phenolics and protein-precipitable tannins were highest in root bark from TT trees and lowest in root bark from TF trees. Biochemical parameters of root bark tissue were regressed with incidence of infection; coefficients of determination (rsuperscript 2) ranged from 0.07 (starch) to 0.57 (phenolic compounds). Ratios of the energetic costs of phenolic and of lignin degradation to the energy available from sugars (Epd:Eas and Eld:Eas) were correlated with incidence of infection (rsuperscript 2 = 0.77 and 0.70, respectively). It is concluded that thinning combined with fertilization may predispose P. menziesii trees to infection by A. ostoyae by lowering concn of defensive compounds in root bark and increasing the energy available to the fungus to degrade them.

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34.
Erickson, R.D. 1995. Douglas-fir tussock moth. In Forest Pest Leaflet 09. Pacific-Forestry-Centre,-Canadian-Forest-Service.

Keywords:      tree/stand protection

                        tree/stand health

Abstract: The recognition, biology, host plants, injuriousness, distribution and control of the lymantriid Orgyia pseudotsugata, especially on Douglas fir (Pseudotsuga menziesii), in British Columbia are discussed. Natural controls, such as parasitoids, predators and a nuclear polyhedrosis virus, normally control endemic populations, and a list is provided of 3 species of parasitic Diptera and 17 Hymenoptera that have been reared from the lymantriid in British Columbia.

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35.
Fashler, A.M.K. and Y.A. El-Kassaby. 1987. The effect of water spray cooling treatment on reproductive phenology in a Douglas-fir seed orchard. Silvae-Genetica 36(5-6): 245-249.